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Part:BBa_K1093024

Designed by: Jakub Piątkowski   Group: iGEM13_Warsaw   (2013-09-23)


MinC/Lysis device-based kill switch with delayed expression

This part combines elements from the well described bacterial lysis device (BBa_K1120808) with the gene coding for MinC protein (cell division inhibitor, characterised by Team Warsaw 2010). Addition of MinC is meant do reduce the odds of individual cells adapting to the device, thus making it suitable for the programmed cell death of bacterial population. In order amplify this effect, expression of lysis device is delayed in relation to MinC. System is under the control of BBa_K098995 heat-inducible input device. The kill-switch is activated in 42 degrees Celsius.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4693
    Illegal NheI site found at 4716
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2006
    Illegal AgeI site found at 4298
    Illegal AgeI site found at 4368
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3278
    Illegal SapI site found at 4949


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Parameters
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